Synthesis of a Heat-stable Enterotoxin (STh) Produced by a Human Strain SK-1 of Enterotoxigenic Escherichia coli

Abstract
A peptide with the amino acid sequence proposed for a heat-stable enterotoxin (STh) produced by a human strain SK-1 of enterotoxigenic Escherichia coli was synthesized by a conventional method. Synthetic STh evoked fluid secretion in suckling mice at a dose of 0.8 ng, which is similar to the effective dose of native STh. The fluid secretion was completely inhibited by antisera raised against the native toxin. Furthermore, synthetic STh showed the same 1H-NMR spectrum and heat-stability as native STh.

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