Identification and Characterization of a Piscine Neuropathy and Nodavirus from Juvenile Atlantic Cod from the Atlantic Coast of North America

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Abstract
In 1999, disease outbreaks in juvenile Atlantic cod Gadus morhua that showed the classic signs of viral encephalopathy and retinopathy (VER) were reported in Nova Scotia. Brain and retinal tissues from moribund cod showed diffuse degenerative vacuolative encephalopathy and degenerative histiocytic retinitis. The affected brain and retinal tissues were observed to be positive for nodaviral antigens by means of immunohistochemical techniques. We partially characterized a nodavirus-like agent from brain and eye tissues and cell culture using reverse-transcriptase polymerase chain reaction and primer sets originally designed for amplification of white trevally Caranx dentex (also known as striped jack Pseudocaranx dentex) and Atlantic halibut Hippoglossus hippoglossus nervous necrosis virus coat (capsid) proteins. Sequencing of the T2 region of the coat protein revealed high similarities (>85% nucleotide identity) to the coat protein genes of other fish nodavirus strains, especially those of Atlantic...