Hormonal control of neurotransmitter choice in sympathetic neurone cultures

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Abstract
Manipulation of the cellular and hormonal environment of cultures of dissociated primary neurons can be used to explore a neuron''s developmental potential and to investigate the factors required for normal development. For example, developing adrenergic sympathetic neurons can be influenced to become cholinergic by both diffusible and membrane-bound factors from certain types of non-neuronal cells; when medium conditioned by incubation on rat heart cell cultures (CM) is placed on the neurons, they develop the ability to produce acetylcholine (ACh) and they form functional cholinergic synapses with each other. Hormones could also contribute to the control of this transmitter choice, and corticosterone treatment of whole superior cervical ganglia (SCG) greatly inhibited the cholinergic development of these ganglia in culture. It was not clear whether the hormone acted directly on the neurons or indirectly via the non-neuronal cells. To study the role of hormones on this transmitter choice, a serum-free medium was developed for the preparation of conditioned medium. Glucocorticoids and epidermal growth factor (EGF) exert dramatic and antagonistic effects on the adrenergic-cholinergic transmitter choice and apparently do so indirectly, by controlling the ability of heart cells to produce cholinergic CM.