Contraction-Free, Fume-Fixed Longitudinal Sections of Fresh Frozen Muscle

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Abstract
Contraction damage occurring when longitudinal frozen sections of fresh unfixed muscles are thawed on microscope slides has limited histological examination of this tissue mainly to cross sections. Longitudinally oriented sections are advantageous for investigating properties that vary along the length of the muscle fibers. A fume fixation technique has been developed for preventing contraction of thick longitudinal frozen aections. The technique is compatible with histochemical staining of enzymes.