Size of primary transcripts in Ehrlich ascites cells as measured by tetraphosphate determination.
- 1 June 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (6) , 1994-1998
- https://doi.org/10.1073/pnas.73.6.1994
Abstract
A method for the quantitation of 5''-tetraphosphate ends in 32P-labeled RNA was developed. The tetraphosphate content of different RNA fractions obtained from Ehrlich ascites cells labeled with 32P for different lengths of time was determined. Ribosomal RNA and poly(U)-binding RNA, labeled for long periods, (mRNA) lack 5''-terminal tetraphosphate. 5 S RNA, pulse labeled 4-5 S RNA, and poly(U)-binding hnRNA (heterogeneous nuclear RNA) do contain tetraphosphate. From the amount of the tetraphosphate, molecular weight data cna be calculated for these RNA fractions which agree with independent determinations by denaturing gel electrophoresis. The majority of the poly(A) containing hnRNA molecules are small (< 28 S) and contain the tetraphosphate of the primary transcript. Therefore, they do not originate from the 3''-end of large molecules by processing events.Keywords
This publication has 35 references indexed in Scilit:
- Animal DNA-dependent RNA polymerases: X. General enzymatic properties of purified calf thymus RNA polymerases AI and BBiochimie, 1974
- 5′‐Ends of Ribosomal and Ribosomal Precursor RNAs from Xenopus laevisEuropean Journal of Biochemistry, 1974
- Organization, Transcription, and Regulation in the Animal GenomeThe Quarterly Review of Biology, 1973
- Gel electrophoresis of RNA under denaturing conditionsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Transcription of chromatin. Initial and terminal nucleotides of ribonucleic acid synthesized by calf thymus and Escherichia coli ribonucleic acid polymerasesBiochemistry, 1973
- Demonstration of Globin Messenger Sequences in Giant Nuclear Precursors of Messenger RNA of Avian ErythroblastsProceedings of the National Academy of Sciences, 1973
- High Stability of Messenger RNA in Growing Cultured CellsNature, 1972
- Transcription of the Polyoma Virus Genome: Synthesis and Cleavage of Giant Late Polyoma-Specific RNAProceedings of the National Academy of Sciences, 1971
- Ribonucleic acids from animal cells.1968
- Localization and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome-associated messenger RNA in HeLa cellsJournal of Molecular Biology, 1968