The Determination of Proteins in Lipid-Rich Mitochondrial Suspensions

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Abstract
Human liver mitochondria contain 2- to 3-fold more lipids than the rat liver mitochondria. This fact produces an important turbidity of samples in protein determinations by the Gornall method, that could not be entirely removed by ether extraction. Parallel estimations by the procedures of Lowry (in this case turbidity does not interfere) and Kjeldahl were done with similar results. Working with rat liver mitochondria, a good concordance in protein content by all 3 methods was obtained. Therefore, for accurate measurements of proteins in lipid-rich mitochondrial suspensions only Lowry’s procedure is suitable.

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