A method for accurate amplification of polymorphic CA-repeat sequences.
Open Access
- 1 August 1993
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 3 (1) , 7-12
- https://doi.org/10.1101/gr.3.1.7
Abstract
Anomalous PCR products are often produced during the amplification of d(CA)n.d(TG)n sequences. Upon denaturing polyacrylamide gel electrophoresis, these products yield a ladder-like pattern that can complicate genotypic interpretation. We have developed two related techniques, referred to as two- and three-stage linear amplification (2-SLA and 3-SLA, respectively), which largely overcome this problem and yield readily interpretable banding patterns.Keywords
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