Ion trap mass spectrometry in the structural analysis of haemoglobin peptides modified by epichlorohydrin and diepoxybutane
- 15 May 2002
- journal article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 16 (9) , 840-847
- https://doi.org/10.1002/rcm.645
Abstract
Ion trap mass spectrometry has been shown to be particularly suitable for the structural analysis of high molecular weight peptides directly fragmented in the mass analyser without needing further sub‐digestion reactions. Here we report the advantages of using multi‐stage ion trap mass spectrometry in the structural characterisation of haemoglobin alkylated with epichlorohydrin and diepoxybutane. Alkylated globins were digested with trypsin and the peptide mixtures were analysed by MS3. This technique allows the sequential fragmentation of peptides under analysis, giving rise to MS3 product ion spectra with additional information with respect to MS2 mass spectra. The results obtained complete the previously reported structural characterisation of alkylated haemoglobin, demonstrating the potential of ion trap mass spectrometry. Copyright © 2002 John Wiley & Sons, Ltd.Keywords
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