Simplified Measurement of Monoglycerides, Diglycerides, Triglycerides, and Free Fatty Acids in Biological Samples
- 1 March 1971
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 17 (3) , 145-147
- https://doi.org/10.1093/clinchem/17.3.145
Abstract
A sensitive (5 to 100 µg) and reproducible (±3.54% SD) method is described for determining monoglycerides, diglycerides, triglycerides, and free fatty acids. After these lipid classes are separated from extracts of plasma or tissues by a single thin-layer chromatographic development, they are eluted into concentrated H2SO4 at 200°C. The resulting color is stable for more than six days.Keywords
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