Water‐soluble 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin complex with human α‐fetoprotein: properties, toxicity in vivo and antitumor activity in vitro
- 30 April 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 450 (1-2) , 49-51
- https://doi.org/10.1016/s0014-5793(99)00440-8
Abstract
The conditions for the formation of a non-covalent complex between 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and the human transport fetal protein, alpha-fetoprotein (AFP), have been studied. TCDD has been shown to form a stable complex with AFP in a 2:1 (TCDD:AFP) ratio. The apparent solubility of TCDD in water increases 10(5)-fold after complex formation. The toxicity of the TCDD:AFP complex injected into mice by the intravenous route is comparable with that of free TCDD administered in oil solution per os. The complex manifests very much higher toxicity (200-1400 times) against human tumor cells (CEM, MCF-7, HepG2) in vitro and surpasses TCDD in selectivity. AFP may facilitate TCDD transport in embryonic tissues and enhance its embryotoxic and teratogenic effects.Keywords
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