Waveguide immunofluorescence sensor for water pollution analysis

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Abstract
A regenerable channel waveguide fluorescence sensor for environmental monitoring is reported. The sensor has been characterized as a detector of the pesticide 2,4 dichlorophenoxyacetic acid. A binding inhibition assay, using fluorescent Cy5.5 dye-labeled antibodies, was monitored at the modified surface of the glass waveguide to detect the target analyte. Three calibration curves were determined and averaged. The averaged calibration curve has a mid-point of 0.68 ppb and a calculated detection limit of 0.28 ppb. Incorporation of a 20 nm thick tantalum pentoxide film at the waveguide surface enhanced the peak fluorescence signal by a factor of approximately 6 compared with an uncoated sensor.© (1998) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.

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