Identification of a receptor for protein import into mitochondria
- 1 October 1990
- journal article
- Published by Springer Nature in Nature
- Vol. 347 (6292) , 444-449
- https://doi.org/10.1038/347444a0
Abstract
Anti-idiotypic antibodies, prepared using a chemically synthesized signal peptide of a mitochondrial precursor protein, recognized a mitochondrial integral membrane protein (p32). Fab fragments derived from both anti-idiotypic antibodies and monospecific antibodies against purified p32 inhibited protein import into mitochondria. Moreover, anti-p32 antibodies specifically immunoprecipitated a precursor-p32 complex after detergent solubilization of mitochondria. Immunoelectron microscopy and subfractionation of mitochondria indicate that p32 is located in contact sites between the outer and inner mitochondrial membranes.Keywords
This publication has 30 references indexed in Scilit:
- Isolation and characterization of the gene for a yeast mitochondrial import receptorNature, 1990
- Purification of the Putative Import-Receptor for the Precursor of the Mitochondrial Protein1The Journal of Biochemistry, 1990
- MOM19, an import receptor for mitochondrial precursor proteinsCell, 1989
- Protein import into mitochondria: ATP-dependent protein translocation activity in a submitochondrial fraction enriched in membrane contact sites and specific proteins.The Journal of cell biology, 1989
- A 42K outer-membrane protein is a component of the yeast mitochondrial protein import siteNature, 1989
- 70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria.The Journal of cell biology, 1988
- Identification of a receptor for protein import into chloroplasts and its localization to envelope contact zonesNature, 1988
- Characterization of translocation contact sites involved in the import of mitochondrial proteins.The Journal of cell biology, 1987
- The cleavable prepiece of an imported mitochondrial protein is sufficient to direct cytosolic dihydrofolate reductase into the mitochondrial matrixFEBS Letters, 1984
- Import of proteins into mitochondria: precursor forms of the extramitochondrially made F1-ATPase subunits in yeast.Proceedings of the National Academy of Sciences, 1979