Effect of delayed refrigeration on plasma factors in whole blood collected in CPDA‐2

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Abstract
Extension of the time within which whole blood may be separated into components offers logistic advantages for the operation of remote mobile drawing teams. The effect of an 8-h hold of whole blood at room temperature before preparation of components was evaluated. Plasma coagulation activity and opsonic factor content were studied in 14 U drawn into the anticoagulant-preservative solution citrate-phosphate-dextrose-adenine (CPDA-2). At the time of collection, an additional 7-ml aliquot was drawn into 1 ml of CPDA-2, and the plasma separated and frozen immediately. Components were prepared from whole blood units allowed to rest undisturbed at 22 .+-. 1.degree. C for 8 h. After 8 h, a significant decrement of .apprx. 10% was found in the concentration of fibrinogen, plasminogen, fibronectin and activity of factor V. Factor VIII activities (VIIIAHF [antihemorrhagic factor] and VIIIAGN [antigen]) were not significantly different after 8 h. Room temperature storage for 8 h before component processing had minimal effects on potentially labile plasma protein factors using CPDA-2 anticoagulant-preservative solution.