MONOCLONAL-ANTIBODIES TO RAT SARCOMATA .1. IMMUNIZATION PROCEDURES AND SOURCE OF LYMPHOID-CELLS FOR HYBRIDOMA PRODUCTION

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 47  (3) , 397-405
Abstract
Hybridomas secreting monoclonal antibodies with specificity for Hooded rat fibrosarcomata were obtained by fusion of the rat myeloma Y3 Ag 1.2.3 with cells taken from spleens or lymph nodes of immunized syngeneic and allogeneic donors. Two of the monoclonal antibodies, both derived from the spleens of tumor bearers, showed specificity for individual tumors, one for MC24 (M10/76) and the other for HSNTC (11/160). These 2 antibodies had a long half-life in the blood when injected i.v. showing that they had a low affinity for normal tissue antigens. Monoclonal antibodies exhibiting broad tumor specificity or extensive cross-reactivity with normal cells were secreted by many of the hybridomas derived from both syngeneic and allogeneic rats that had been hyperimmunized with tumor cells. The results are discussed in relation to the production of monoclonal anti-tumor antibodies for use in experimental therapy.