ARTIFACTS IN FLUORESCENCE EMISSION SPECTROSCOPY RELATED TO WOOD'S ANOMALY

1 September 1991

journal article
Published by Wiley in Photochemistry and Photobiology

Vol. 54 (3) , 481-483
https://doi.org/10.1111/j.1751-1097.1991.tb02045.x

Abstract

Fluorescence emission spectra of porphyrins acquired with a monochromator using a 1500 line/mm holographic grating were affected by polarization-induced effects (Wood's anomaly) which affected the transmission of different wavelengths of light. This effect resulted in a blue-shift in the emission optimum, and distortions in the fluorescence spectrum at longer wavelengths. These anomalies could be eliminated by insertion of polarizers, orientation = 0 degrees, in emission and excitation paths. A monochromator with a 1200 line/mm holographic grating was essentially free from these distortions.

Keywords

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