Adsorption, in vitro, de l’endopolygalacturonase secrétée par Colletotrichum lindemuthianum sur des parois de deux lignées de haricots isogéniques résistants et sensibles à l'anthracnose

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Abstract
As compared with the cell walls of anthracnose-sensitive beans, the cell walls of resistant beans, with the involvement of Cornell gene, adsorb, when cultivated in vitro, larger quantities of the endopolygalacturonase secreted by the fungus responsible for the disease, Colleotrichum lindemuthianum. This selective adsorption only occurs for the enzyme excreted by the pathogenic fungus. This phenomenon is observed with cell walls from which the constituant glycoproteins inhibitive for the endopolygalacturonase were previously removed. The adsorbing capacity of the two types of cell walls was reduced by a heat treatment (100 °C, 30 min) and the differences between both types of walls for the selective binding with the endopolygalacturonase of C. lindemuthianum are reduced. This suggests that protein(s) are involved in this specific adsorption. Solubilisation of the pectic substances of the walls by an enzymatic treatment strongly reduced the binding capacity of the walls for the endopolygalacturonase. It appears therefore that ionic bindings with the pectic polymers are also involved. The possible implication of this phenomenon in the resistance to anthracnose is discussed. [Translated by the Journal]

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