Abstract
Addition of 4–40 μm 2,4-dichlorophenoxyacetic acid (2,4-D) plus 2–6 μm kinetin induced callus formation in fresh cotyledons of walnut (Juglans regia L.), but the presence of phenolic compounds prevented a stable culture. Roots were obtained from callus on a half-strength K(h) medium supplemented with indolebutyric acid (IBA) or naphthaleneacetic acid (NAA) at 40 μm plus kinetin or benzylamino purine (BA) at 2 μm. The origin of roots was usually endogenous.

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