AN ANALYSIS OF FROZEN SECTION TECHNIQUES: I. SECTIONING OF FRESH-FROZEN TISSUE
Open Access
- 1 January 1957
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 5 (1) , 47-52
- https://doi.org/10.1177/5.1.47
Abstract
Optimal Conditions for cutting thin sections from fresh-frozen tissue have been described in terms of a set of optimal temperatures for the tissue block, microtome chamber, and knife. A model for the cutting process is proposed which postulates a zone of "micro-melting" at the edge of the knife. The behavior of the freezing microtome is interpreted in terms of this model for a variety of conditions.Keywords
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- LOCALIZATION OF ANTIGEN IN TISSUE CELLSThe Journal of Experimental Medicine, 1951
- The Rapid Preparation of Frozen Tissue SectionsStain Technology, 1948