The Splenic Suppressor Cell

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Abstract
3H-thymidine incorporation was measured in mixed lymphocyte cultures of allogeneic rat spleen cells, the stimulating (donor) cells having been treated with mitomycin C (MC). Removal of adherent cells from either stimulating or reactive cells, or both, by passage through a glass wool column gave a substantial elevation of the response. The effect of such treatment of the stimulating cells could be duplicated by treating them with cycloheximide or by use of cells from doubly thymus-deprived donors. MC treatment, on the other hand, did not affect their supporessor activity. It was inferred that a subpopulation of splenic T cells adherent to glass wool act as suppressors in this immunologic system and that their action requires protein synthesis but not cell division. A close similarity was noted between these suppressor cells and those active in earlier experiments with PHA and concanavalin A and with suppressor cells studied in intact animals subjected to the graft-vs-host reaction. A general classification of suppressor cell systems is proposed.

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