INHIBITOR OF HEPATOMA-CELLS MULTIPLICATION IN EFFERENT FLUID FROM ISOLATED PERFUSED RAT-LIVER

  • 1 January 1975
    • journal article
    • research article
    • Vol. 23  (10) , 434-437
Abstract
Efferent fluid from isolated rat livers perfused with a synthetic medium, when concentrated, inhibits the multiplication of hepatoma LF 20 cells in vitro. The inhibitory factor was thermolabile and inactivated at a slow rate by proteolytic enzymes. Sephadex and acrylamide agarose chromatographic fractionation yielded a MW around 100,000 for the inhibitory factor. The release of the factor by the perfused liver depended on the temperature of the perfusing fluid. The potential role of this factor in the control of liver cell proliferation was discussed.

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