Point mutations in the 23 S rRNA genes of four lincomycin resistant Nicotiana plumbaginifolia mutants could provide new selectable markers for chloroplast transformation
- 1 October 1988
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 214 (2) , 295-299
- https://doi.org/10.1007/bf00337724
Abstract
Experiments designed to establish stable chloroplast transformation require selectable marker genes encoded by the chloroplast genome. The antibiotic lincomycin is a specific inhibitor of chloroplast ribosomal activity and is known to bind to the large ribosomal subunit. We have investigated a defined region of the chloroplast 23 S rRNA genes from four lincomycin resistant Nicotiana plumbaginifolia mutants and from wild-type N. plumbaginifolia. The mutants LR415, LR421 and LR446 have A to G transitions at positions equivalent to the nucleotides 2058 and 2059 in the Escherichia coli 23 S rRNA. The mutant, LR400, possesses a G to A transition at a position corresponding to nucleotide 2032 of the E. coli 23 S rRNA.Keywords
This publication has 43 references indexed in Scilit:
- Chloramphenicol, erythromycin, carbomycin and vernamycin B protect overlapping sites in the peptidyl transferase region of 23S ribosomal RNABiochimie, 1987
- Decoding at the ribosomal A site: antibiotics, misreading and energy of aminoacyl-tRNA bindingBiochimie, 1987
- A point mutation in the chloroplast 16 S rRNA gene of a streptomycin resistant Nicotiana tabacumFEBS Letters, 1987
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985
- Ribosome structure: binding site of macrolides studied by photoaffinity labelingBiochemistry, 1985
- Isolation and Characterization of Mutants in Plant Cell CultureAnnual Review of Plant Physiology, 1984
- Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas.The Journal of cell biology, 1983
- Altered ribosomal RNA genes in mitochondria from mammalian cells with chloramphenicol resistanceNature, 1981
- Sequence of the intron and flanking exons of the mitochondrial 21S rRNA gene of yeast strains having different alleles at the ω and rib-1 lociCell, 1980
- Substrate‐and Antibiotic‐Binding Sites at the Peptidyl‐Transferase Centre of Escherichia coli RibosomesEuropean Journal of Biochemistry, 1971