Porcine Sugar Nucleotide: Glycoprotein Glycosyltransferases. III. Blood Serum and Liver N-Acetylglucosaminyltransferase

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Abstract
Pork liver and serum contain UDP-N-acetylglucosamine: glycoprotein N-acetylglucosaminyltransferase activity. The serum enzyme is soluble and the liver enzyme is membrane-bound; the latter can be solubilized by organic solvent extraction but the yield is low and the soluble preparation is unstable. Comparison of membrane-bound and soluble liver enzymes indicates that the membrane has only a minor modifying effect on the enzyme kinetics. Comparison of serum and liver enzymes shows that the two activities are similar but not identical; in particular, the two enzymes have similar cation requirements, substrate specificities, pH profiles, and Km values for both nucleotide sugar and glycoprotein acceptor. The source and function of the serum enzyme cannot be deduced from this information.

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