Synthesis of substrate analogues for trypanothione reductase
- 1 February 1993
- journal article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 41 (2) , 141-146
- https://doi.org/10.1111/j.1399-3011.1993.tb00124.x
Abstract
The synthesis and chemical characterisation of a range of substrate analogues for trypanothione reductase are described, with the spermidine portion of trypanothione replaced by the 3-dimethylaminopropylamide moiety. Using 1-hydroxybenzotriazole/N-hydroxysuccinimide coupling, products were obtained which had a range of replacements of the gamma-glutamyl groups of the enzyme substrate. The materials were characterised by FPLC, 1H/13C NMR spectroscopy and FAB mass spectroscopy.Keywords
This publication has 13 references indexed in Scilit:
- Synthesis of N-benzyloxycarbonyl-l-cysteinylglycine 3-dimethylaminopropylamide disulfide: A cheap and convenient new assay for trypanothione reductaseAnalytical Biochemistry, 1991
- "Subversive" substrates for the enzyme trypanothione disulfide reductase: alternative approach to chemotherapy of Chagas disease.Proceedings of the National Academy of Sciences, 1988
- Substrate specificity of the flavoprotein trypanothione disulfide reductase from Crithidia fasciculataBiochemistry, 1987
- Synthesis of the trypsin fragment 10-25/75-88 of mouse nerve growth factor*International Journal of Peptide and Protein Research, 1987
- Purification and characterization of trypanothione reductase from Crithidia fasciculata, a new member of the family of disulfide-containing flavoprotein reductasesBiochemistry, 1986
- Trypanothione: A Novel Bis(glutathionyl)spermidine Cofactor for Glutathione Reductase in TrypanosomatidsScience, 1985
- A new -butyloxycarbonylating reagent, 2--butyloxycarbonyloxyimino-2-phenylacetoni trile.Tetrahedron Letters, 1975
- Notiz zur Entacylierung von N‐Sulfenyl‐peptiden mittels Rhodanid‐IonenEuropean Journal of Inorganic Chemistry, 1972
- Eine neue Methode zur Synthese von Peptiden: Aktivierung der Carboxylgruppe mit Dicyclohexylcarbodiimid unter Zusatz von 1‐Hydroxy‐benzotriazolenEuropean Journal of Inorganic Chemistry, 1970
- Amino acids and peptides. LXXXIV. Alcoholytic removal of o-nitrophenylsulphenyl protecting groups; Synthesis of simple peptides without isolation of intermediatesCollection of Czechoslovak Chemical Communications, 1968