Infection and transformation of fresh human umbilical cord blood cells by multiple sources of human T‐cell leukemia‐lymphoma virus (HTLV)

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Abstract
Human T‐cell leukemia‐lymphoma virus (HTLV) was first isolated from sporadic patients with adult T‐cell malignancies in the United States and subsequently from T‐lymphocytes established in culture from additional T‐cell leukemia‐lymphoma patients living in different geographical areas of the world. Co‐cultivation of normal unbilical cord blood with lethally irradiated, HTLV‐positive lymphocytes established in culture from many of these patients resulted in the productive infection of the cord blood T‐lymphocytes which grew in suspension culture in the absence of exogenous TCGF. These transformed cord blood cells have morphological and cytochemical properties similar to HTLV‐positive fresh and cultured tumor T‐cells and are distinguishable from virus donor cells by HLA haplotype and chromosomal markers. These cells express HTLV proteins, release type‐C virus particles and contain surface receptors for TCGF. These results demonstrate that HTLV isolated from T‐cell leukemic donors from different parts of the world can productively infect and transform fresh human cord blood T‐lymphocytes, and that the transformed cells share many similarities with fresh or cultured leukemic cells.

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