Biochemical and genetic analysis of hydrogen metabolism in Escherichia coli: the hydB gene
Open Access
- 1 December 1988
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 170 (12) , 5433-5439
- https://doi.org/10.1128/jb.170.12.5433-5439.1988
Abstract
Production of active hydrogenase by Escherichia coli requires several gene products. One of the essential genes, hydB, is encoded by a DNA fragment of approximately 1.0 kilobase. The hydB gene produced a protein with an apparent molecular weight of 32,000. The hydB gene was transcribed only under anaerobic conditions. Oxygen and nitrate repressed transcription of this gene. hydB gene transcription also required sigma 60, the product of the rpoN gene.This publication has 57 references indexed in Scilit:
- Involvement of the ntrA gene product in the anaerobic metabolism of Escherichia coliMolecular Genetics and Genomics, 1987
- Isolation of Genes Required for Hydrogenase Synthesis in Escherichia coliMicrobiology, 1987
- Isolation and characterisation of a soluble active fragment of hydrogenase isoenzyme 2 from the membranes of anaerobically grown Escherichia coliEuropean Journal of Biochemistry, 1986
- Purification and properties of membrane-bound hydrogenase isoenzyme 1 from anaerobically grown Escherichia coli K12European Journal of Biochemistry, 1986
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Molecular cloning and physical mapping of the hyd gene ofEscherichia coliK-12FEMS Microbiology Letters, 1984
- Mutants of Escherichia coli with Altered Hydrogenase ActivityMicrobiology, 1984
- Physiological effects of dissolved oxygen tension and redox potential on growing populations of micro-organismsJournal of Chemical Technology & Biotechnology, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Characterization by in vitro complementation of a peptide corresponding to an operator-proximal segment of the β-galactosidase structural gene of Escherichia coliJournal of Molecular Biology, 1967