The corrinoid from Methanobacterium thermoautotrophicum (Marburg strain). Spectroscopic structure analysis and identification as Cobeta-cyano-5'-hydroxybenzimidazolyl-cobamide (factor III)

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Abstract
The corrinoids from Methanobacterium thermoautotrophicum were extracted as the Co-cyano derivative, which was isolated in crystalline form. A consistent set of spectroscopic data was acquired (ultraviolet/visible, circular dichroic, infrared, fast-atom-bombardment mass, 1H-NMR and 13C-NMR spectra), which allowed the structural analysis of this complete corrinoid. It was assigned the structure of the Co.beta.-cyano-5''-hydroxybenzimidazolyl-cobamide and was identified with Friedrich and Bernhauer''s ''factor III'' by comparison with an authentic sample.