Effects of Iodoacetamide and X Rays on a Murine Ascites Tumor

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Abstract
The radiosensitizing properties of iodoacetamide (IA) were studied using as end point the proliferative capacity of an ascites tumor (lymphoma, LSA) of the C57BL mouse. When the cells were irradiated in vitro, TD50 [median total dose] assays gave a survival curve with Do = 120 .+-. 20 rad and n = 4.0. When cells were irradiated in the presence of 10-3 M iodoacetamide (IA), the shoulder of the curve was eliminated, but the slope was not changed. In vivo-in vivo experiments were performed using a 7 day old tumor. The cells were treated in the donor mice, which were injected i.p. with 104 cells 7 days before treatment. Survival curves for X rays along gave Do = 369 .+-. 50 rad, with n = 1.70. Irradiation after injection of 0.37 mg of IA eliminated the shoulder and changed the slope (Do = 243 .+-. 35 rad). The ratio of the Do gave a slope ratio of 1.52 .+-. 0.32, indicating a sensitizing action of IA. Using the potentiating factor (PF), defined as the ratio of the expected to the experimental surviving fraction of the combination, an average PF = 5.61 was obtained. PF 1.0 indicate a synergistic effect of IA and X-rays.

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