Localization and DNA sequence around the initiation site of ribosomal RNA transcription inPhysarum polycephalum
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (23) , 8519-8533
- https://doi.org/10.1093/nar/11.23.8519
Abstract
We have used S1 nuclease to map the initiation site of ribosomal RNA transcription in the acellular slime mold Physarum polycephalum, and we have determined the sequence of 1011 nucleotides surrounding the start site. Consistent with others' observations, there is little homology with the comparable region of other species. As predicted by previous restriction mapping, direct repeats roughly 30 base pairs in length are present upstream of the initiation site and a 148 base pair duplication occurs in the external transcribed spacer. The results also suggest the presence of a processing site within the external transcribed spacer of the ribosomal transcription unit.Keywords
This publication has 49 references indexed in Scilit:
- Transcription of cloned Xenopus laevis ribosomal DNA microinjected into Xenopus oocytes, and the identification of an RNA polymerase I promoterCell, 1982
- Structure of the central spacer region of extrachromosomal ribosomal DNA in Physarum polycephalumJournal of Molecular Biology, 1982
- Nucleotide sequence of the transcription initiation region of a rat ribosomal RNA geneGene, 1982
- Structure around the 3' terminus of the 26S ribosomal RNA gene of Physarum polycephalumGene, 1981
- A split promoter for a eucaryotic tRNA geneCell, 1981
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3′ border of the regionCell, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5′ border of the regionCell, 1980
- Initiation of transcription of ribosomal deoxyribonucleic acid sequences in isolated nuclei of Physarum polycephalum: studies using nucleoside 5'-[.gamma.-S]triphosphates and labeled precursorsBiochemistry, 1979
- The intervening sequence in the 26s rrna coding region of t. thermophila is transcribed within the largest stable precursor for rRNACell, 1979
- Structure of ribosomal DNA in Physarum polycephalumJournal of Molecular Biology, 1976