A METHOD FOR COUNTING LEUKOCYTES IN FILTERED COMPONENTS
- 1 May 1990
- journal article
- research article
- Published by Wiley in Transfusion
- Vol. 30 (4) , 377-379
- https://doi.org/10.1046/j.1537-2995.1990.30490273449.x
Abstract
Leukocyte-depleted components are being used more frequently. Quantitation of residual WBCs is needed for quality control and for establishing the critical level required to prevent alloimmunization. Electronic counting is not accurate at WBC levels below 100/.mu.L (1) and previously proposed techniques are either tedious ore require sophisticated equipment. We describe a simple hemacytometer procedure using Turks solution that gives 87-93% recovery at WBC levels below 40/.mu.L.This publication has 6 references indexed in Scilit:
- A flow cytometric technique to accurately measure post‐filtration white blood cell countsTransfusion, 1989
- Effectiveness of two synthetic fiber filters for removing white cells from AS‐1 red cellsTransfusion, 1989
- Transfusion-Induced Alloimmunization and Immunosuppression and the Effects of Leukocyte DepletionTransfusion Medicine Reviews, 1989
- Quality control and evaluation of leukocyte-depleting filtersTransfusion, 1989
- Ficoll-isopaque gradients for the determination of density distributions of human blood lymphocytes and other reticulo-endothelial cellsExperimental Cell Research, 1974
- Changes in the carbohydrate metabolism of mitogenicellay stimulated human peripheral lymphocytes I. Stimulation by phytohaemagglutininBiochimica et Biophysica Acta (BBA) - General Subjects, 1970