Classification of Human Adenoviruses by SDS-Polyacrylamide Gel Electrophoresis of Structural Polypeptides

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Abstract
The virion polypeptide compositions of 15 human adenovirus types, representing all members of subgroups B and C and selected members of subgroups A and D, were analyzed by SDS-PAGE [sodium dodecyl sulfate polyacrylamide gel electrophoresis]. The capsid polypeptides II, III, IIIa and IV displayed a pattern of apparent MW which was characteristic for each adenovirus serotype analyzed. Adenovirus serotypes belonging to the same subgroup shared an apparent MW pattern of the internal structural polypeptides in the following manner: Subgroup A: V.sbd.resolved into 2 bands of 46-48.5 K [kilodaltons] and 51-51.5 K; VI.sbd.25.5 K; and VII.sbd.18 K. Subgroup B: V.sbd.53.5-54.5 K; VI.sbd.24 K; and VII.sbd.18 K. Subgroup C: V.sbd.48.5 K; VI.sbd.24 K; and VII.sbd.18.5 K. Subgroup D: V.sbd.50.5 K; VI.sbd.23.5 K; and VII.sbd.18 K. Adenovirus type 4: V.sbd.48 K; VI.sbd.24.5 K; and VII.sbd.18 K. The structural polypeptides represent a major portion of the adenovirus gene products. The apparent MW differences of viral polypeptides should be used to confirm the identification of an adenovirus type and for subgroup classification of adenoviruses. Adenovirus type 4 should be classified as a member of a separate subgroup designated E.

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