Production of antibody against T-2 toxin
- 1 January 1979
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 37 (1) , 104-108
- https://doi.org/10.1128/aem.37.1.104-108.1979
Abstract
Antibody against T-2 toxin was obtained after immunization of rabbits with bovine serum albumin-T-2 hemisuccinate conjugate. The antibody had greatest binding efficiency for T-2 toxin, less efficiency for HT-2, and least for T-2 triol. Cross-reaction of antibody with neosolaniol, T-2 tetraol, and 8-acetyl-neosolaniol was very weak. Diacetoxyscirpenol, trichodermin, vomitoxin, and verrucarin A essentially gave no cross-reaction with the antibody. The sensitivity of the binding assay for T-2 toxin detection was in the range of 1 to 20 ng per assay. Detailed methods for the preparation of the conjugate and the production of immune serum and methods for antibody determination are described.This publication has 7 references indexed in Scilit:
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