Cloning and analysis of pif, replication and leading regions of the F plasmid
- 1 October 1984
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 197 (1) , 129-136
- https://doi.org/10.1007/bf00327933
Abstract
We describe the molecular cloning of BglII fragments of the hybrid plasmid pRS5 (pSC101 and EcoRI fragments of F; f7, f5, f3 and f6). The clones isolated were examined for the expression of F-specified replication, incompatibility, mobilization and inhibition of T7 bacteriophage multiplication. Proteins directed by the BglII clones were labelled in Escherichia coli K12 maxicells and analyzed by SDS-polyacrylamide gel electrophoresis. The sizes of previously reported proteins, encoded by the replication, incompatibility and leading regions encompassed by these plasmids have been confirmed in this study. In addition, the results demonstrate that a pif gene, which encodes an 80,000 dalton polypeptide essential for the inhibition T7 phage multiplication, is located on the BglII fragment that spans the junction of EcoRI fragments f7 and f5.This publication has 65 references indexed in Scilit:
- Control of Tn5 transposition in Escherichia coli is mediated by protein from the right repeatCell, 1982
- An Mr 29 000 protein is essential for mini-F maintenance in E. coliGene, 1982
- Cloning of F DNA fragments carrying the origin of transfer oriT and the fertility inhibition gene finPJournal of Molecular Biology, 1981
- Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmidGene, 1981
- Direct repeats of the F plasmid incC region express F incompatibilityCell, 1981
- THE CONJUGATION SYSTEM OF F-LIKE PLASMIDSAnnual Review of Genetics, 1980
- F factor mobilization of non-conjugative chimeric plasmids in Escherichia coli: General mechanisms and a role for site-specific recA-independent recombination at oriV1Journal of Molecular Biology, 1980
- Conjugation proteins encoded by the F sex factorNature, 1977
- Increase in Conjugational Transmission Frequency of Nonconjugative PlasmidsScience, 1977
- Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: II. Structure of drug resistance (R) factors and f factorsJournal of Molecular Biology, 1973