Recognition of UUN codons by two leucine tRNA species from Escherichia coli
Open Access
- 9 May 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 344 (1) , 31-34
- https://doi.org/10.1016/0014-5793(94)00354-8
Abstract
Codon recognition by Escherichia coli tRNALeu 4 and tRNALeu 5 was investigated by analysis of the competition between two aminoacyl‐tRNA species in an in vitro protein synthesis. Both tRNA species strictly obey the wobble rule when they are in competition with other tRNA species. This is probably due to the post‐transcriptional modifications at the first position of the anticodon of these tRNALeu species, supporting the proposal that the conformational rigidity of post‐transcriptionally modified pyrimidine nucleotides guarantees the correct codon recognition.Keywords
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