Interaction of tetraiodofluorescein with a modified form of aspartate transcarbamylase.
- 1 January 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (1) , 111-114
- https://doi.org/10.1073/pnas.74.1.111
Abstract
Low concentrations of the dye tetraiodofluorescein activate native aspartate transcarbamylase (aspartate carbamoyltransferase, carbamoylphosphate:L-aspartate carbamoyltransferase, EC 2.1.3.2), while high concentrations inhibit the enzyme''s activity. This produced similar effects upon a modified form of aspartate transcarbamylase produced by Escherichia coli grown in a culture medium supplemented with thiouracil. The ATP-induced activation is reduced in the modified form of the enzyme to the same extent as is the tetraiodofluorescein-induced activation. Thus, a relationship is demonstrated between the internal mechanisms by which ATP and tetraiodofluorescein activate aspartate transcarbamylase.This publication has 10 references indexed in Scilit:
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