THE INACTIVATION OF TRYPSIN BY ULTRAVIOLET LIGHT, II. THE INVOLVEMENT OF INTRAMOLECULAR HYDROGEN BOND DISRUPTION
- 1 November 1961
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 47 (11) , 1733-1741
- https://doi.org/10.1073/pnas.47.11.1733
Abstract
Ultraviolet inactivation of trypsin was shown to result in the formation of at least three types of trypsin molecules in solution after irradiation. From data on the temperature dependence of the rate of inactivation and activation energies it was possible to specify the manner in which hydrogen bonds are involved. Evidence was presented for the implication of excited triplet states as essential intermediates in the inactivation process.Keywords
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