Expression of ORF A1 of infectious bursal disease virus results in the formation of virus-like particles.
- 1 May 1998
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 79 (5) , 1047-1054
- https://doi.org/10.1099/0022-1317-79-5-1047
Abstract
A recombinant vaccinia virus inducibly expressing ORF A1 of infectious bursal disease virus (IBDV) has been constructed and characterized. Cells infected with this recombinant virus express the IBDV polyprotein, which is proteolytically processed to give mature VP2, VP3, and VP4 polypeptides. An electron microscopy study revealed that the cytoplasm of cells infected with the recombinant virus contains abundant IBDV-like particles (VLP). These VLP form close-packed paracrystalline arrays that are specifically recognized by anti-IBDV antibodies. The size and morphology of purified VLP were found to be akin to those of authentic IBDV particles.Keywords
This publication has 0 references indexed in Scilit: