Leucocyte Alkaline Phosphatase Elevation in Human Acute Leukaemia Derived Cell Lines Cultured in Diffusion Chambers

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Abstract
Leukocyte alkaline phosphatase (LAP) was histochemically detected in 7-18% of cells in tissue culture lines derived from the peripheral blood or bone marrow of each of 5 patients with untreated acute myelogenous or monomyelogenous leukemia and 30% of cells in a clonal line of a rat promyelocytic leukemia. Following transfer to diffusion chambers i.p. implanted into total body irradiated rats, LAP levels were detected in up to 92% of human and 80% of rat leukocytes. There was no associated morphologic differentiation. In rat leukemia cells peroxidase and myeloid specific esterase also increased from tissue culture levels. Return of cells to tissue culture decreased enzymes to preimplant levels. Addition of plasma or peritoneal fluid from irradiated rats to cells in tissue culture again induced LAP. LAP was not increased under these conditions with cell lines derived from patients with acute lymphatic leukemia or Sezary cell leukemia. These studies indicate that a humoral factor in peritoneal fluid and plasma of irradiated rats increases LAP in human and rat leukocytes.