Rapid Real-Time PCR for Determination of Penicillin Susceptibility in Pneumococcal Meningitis, Including Culture-Negative Cases
Open Access
- 1 February 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 40 (2) , 682-684
- https://doi.org/10.1128/jcm.40.2.682-684.2002
Abstract
A novel real-time PCR-hybridization assay was developed for the rapid (Streptococcus pneumoniae. When applied to 24 pneumococcal DNA-positive cerebrospinal fluid extracts, penicillin-sensitiveS. pneumoniaewas detected in all instances. Real-time PCR proved more sensitive than culture, microscopy, or antigen detection and provided susceptibility data even in culture-negative cases.Keywords
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