New Approach for High-Throughput Screening of Drug Activity onPlasmodiumLiver Stages
- 1 April 2006
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 50 (4) , 1586-9
- https://doi.org/10.1128/aac.50.4.1586-1589.2006
Abstract
Plasmodiumliver stages represent potential targets for antimalarial prophylactic drugs. Nevertheless, there is a lack of molecules active on these stages. We have now developed a new approach for the high-throughput screening of drug activity onPlasmodiumliver stages in vitro, based on an infrared fluorescence scanning system. This method allowed us to count automatically and rapidlyPlasmodium-infected hepatocytes, using different hepatic cells and differentPlasmodiumspecies, includingPlasmodium falciparum. This new technique is well adapted for high-throughput drug screening and should facilitate the identification of new antimalarial compounds active onPlasmodiumliver stages.Keywords
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