A simple method is described for the preparation and use of stripping film of high quality for electron microscopic applications of autoradiography. The advantages of the method are: (a) uniform monolayers of emulsion are easily prepared with an inexpensive mechanical device and applied to thin sections on conventional grids; (b) both sides of grids can be coated with emulsion, permitting a potential doubling of sensitivity with, theoretically, no loss of resolution; and (c) large numbers of grids can be processed simultaneously, assuring identical conditions of development.