Extractionless HPLC Method for the Determination of Naproxen in Human Plasma and Urine
- 1 November 1993
- journal article
- bioanalytical
- Published by Taylor & Francis in Analytical Letters
- Vol. 26 (11) , 2341-2348
- https://doi.org/10.1080/00032719308017474
Abstract
A simple and rapid (extractionless) high-performance liquid chromatographic method with ultraviolet detection, at 278 nm, is described for the determination of naproxen in human plasma and urine. Niflumic acid is used as internal standard. The chromatographic system consists of a reversed-phase C18-Spherisorb column with acetonitrile/0.1 M sodium acetate (35:65 v/v, pH 6.14) as the mobile phase. The retention time is 3.0 min for naproxen and 3.8 min for niflumic acid. The total run time is 5 min and the typical assay time is 10 min. The method is sufficiently sensitive for biopharmaceutical studies, after the oral administration of a single sustained release dose.Keywords
This publication has 20 references indexed in Scilit:
- Simultaneous analysis of flunixin, naproxen, ethacrynic acid, indomethacin, phenylbutazone, mefenamic acid and thiosalicycic acid in plasma and urine by high-performance liquid chromatography and gas chromatography—mass spectrometryJournal of Chromatography B: Biomedical Sciences and Applications, 1991
- Liquid chromatography with amperometric detection for the determination of non-steroidal anti-inflammatory drugs in plasmaJournal of Chromatography B: Biomedical Sciences and Applications, 1990
- Identification and simultaneous determination of non-steroidal anti-inflammatory drugs using high-performance liquid chromatographyJournal of Chromatography B: Biomedical Sciences and Applications, 1989
- High-performance liquid chromatographic determination of ketoprofen and naproxen in rat plasmaJournal of Chromatography B: Biomedical Sciences and Applications, 1988
- An Isocratic High-Pressure Liquid Chromatographic Determination of Naproxen and Desmethylnaproxen in Human PlasmaJournal of Pharmaceutical Sciences, 1982
- A High-Performance Liquid Chromatographic Method for the Quantitative Determination of Naproxen and Des-Methyl-Naproxen in Biological SamplesJournal of Liquid Chromatography, 1982
- Quantitative determination of naproxen in plasma by a simple high-performance liquid chromatographic methodJournal of Chromatography B: Biomedical Sciences and Applications, 1981
- Convenient and sensitive high-performance liquid chromatography assay for ketoprofen, naproxen and other allied drugs in plasma or urineJournal of Chromatography A, 1980
- Determination of some anti-inflammatory drugs in serum by high-performance liquid chromatographyJournal of Chromatography A, 1979
- Bioanalysis of Naproxen by High Performance Reversed Phase Liquid Chromatography with Photometric and Fluorimetric DetectionJournal of Liquid Chromatography, 1979