An Improved Analytical Method, Based on HPLC with Electrochemical Detection, for Monitoring Exposure to 3-Chloro-4-fluoroaniline
- 1 November 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Analytical Toxicology
- Vol. 12 (6) , 330-333
- https://doi.org/10.1093/jat/12.6.330
Abstract
An HPLC method for monitoring exposure of workers to 3-chloro-4-fluoroaniline (CFA) is described. It is based on the detection of a major urinary metabolite, 2-amino-4-chloro-5-fluorophenyl sulphate, and is superior to the previously adopted GC method. The limit of detection for the metabolite, 2-amino-4-chloro-5-fluorophenyl sulphate, is less than or equal to 0.01 mg/L (as CFA equivalent), with a coefficient of variation obtained over the range 1–15 mg/L of 4%. The HPLC method avoids the need for derivatization (cf. GC) and is consequently a much quicker and cheaper monitoring method. Urine samples from plant workers may be stored at 4°C for a period of three months without loss of the metabolite.This publication has 0 references indexed in Scilit: