High Pressure Liquid Chromatographic Determination of Vitamin D3 in Instant Nonfat Dried Milk
- 1 September 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 63 (5) , 1163-1167
- https://doi.org/10.1093/jaoac/63.5.1163
Abstract
Vitamin D3 was determined in commercially fortified instant nonfat dried milk by using normal phase high pressure liquid chromatography (HPLC). The sample was extracted with dichloromethane with sodium phosphate tribasic solution added. The sample was cleaned up by using a Sep-Pak silica cartridge and then a microparticulate column containing 10 μm Partisil-10 PAC packing material. The final analysis was performed by using a normal phase HPLC system with a 10 μm LiChrosorb NH2 column. Recovery of vitamin D3 at levels as low as 1000 IU/kg was 97.7% with a standard deviation of 3.9%.This publication has 2 references indexed in Scilit:
- Reverse Phase High Pressure Liquid Chromatographic Determination of Vitamin D in Fortified MilkJournal of AOAC INTERNATIONAL, 1978
- High Pressure Liquid Chromatographic Determination of Vitamin D in Fortified MilkJournal of AOAC INTERNATIONAL, 1977