Fertilization of Steelhead Trout (Salmo gairdneri) Eggs With Cryo-preserved Sperm
- 1 December 1971
- journal article
- Published by Canadian Science Publishing in Journal of the Fisheries Research Board of Canada
- Vol. 28 (12) , 1915-1918
- https://doi.org/10.1139/f71-289
Abstract
The average percent fertilization of fresh steelhead trout (Salmo gairdneri) ova inseminated with cryo-preserved sperm was 59 in 90 tests. Sodium bicarbonate, lecithin, and mannitol at 750, 750, and 250 mg/100 ml, respectively, were included in the extender. The most effective concentration of dimethyl sulfoxide in the extender was 12% (v/v). Significant differences in percent of fresh ova fertilized with cryo-preserved sperm were attributed to both parents.Keywords
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