Site-specific cleavage of single-stranded DNAs at unique sites by a copper-dependent redox reaction
Open Access
- 1 September 1988
- journal article
- letter
- Published by Springer Nature in Nature
- Vol. 335 (6186) , 186-188
- https://doi.org/10.1038/335186a0
Abstract
Metal ions play a crucial role not only in the formation and maintenance of nucleic acid structure, but also in important biochemical conversions of polynucleotides. Some aqueous metal ions, acting as general acid/base (or electrophilic/nucleophilic) catalysts, can induce site-specific cleavage of RNA1–6. DNA is not cleaved efficiently by the non-redox metal-induced mechanism7. However, DNA degradation by radicals formed in the metal-catalysed auto-oxidation of ascorbate (or other reducing agents) is well known8–11. In the past, the observed cleavage reactions have not been very specific. Here, we report a non-enzymatic cleavage of single-stranded DNA occurring at unique sites due to redox reactions involving copper. This could be considered a 'self-cleavage' reaction, by analogy with the lead-induced non-redox RNA cleavage reaction3–5. This site-specific cleavage of DNA, stimulated by ascorbate and hydrogen peroxide, is most efficient under physiological conditions, so this phenomenon may have biological significance.Keywords
This publication has 29 references indexed in Scilit:
- Metal-activated hydrolytic cleavage of DNAJournal of the American Chemical Society, 1987
- Metal ion requirements and other aspects of the reaction catalyzed by M1 RNA, the RNA subunit of ribonuclease P from Escherichia coliBiochemistry, 1986
- Specific Cleavages of DNA by Ascorbate in the Presence of Copper Ion or Copper ChelatesThe Journal of Biochemistry, 1985
- Crystallographic and biochemical investigation of the lead(II)-catalyzed hydrolysis of yeast phenylalanine tRNABiochemistry, 1985
- DNA-Scission Activities of Ascorbate in the Presence of Metal Chelates1The Journal of Biochemistry, 1984
- Lead Ion Binding and RNA Chain Hydrolysis in Phenylalanine tRNAJournal of Biomolecular Structure and Dynamics, 1983
- DNA- and Protein-Scission Activities of Ascorbate in the Presence of Copper Ion and a Copper-Peptide ComplexThe Journal of Biochemistry, 1983
- Pb(II)-catalysed cleavage of the sugar–phosphate backbone of yeast tRNAPhe—implications for lead toxicity and self-splicing RNANature, 1983
- Effect of europium(III) on the thermal denaturation and cleavage of transfer ribonucleic acidsBiopolymers, 1976
- Specific cleavages of pure tRNAs by plumbous ionsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976