SPIROADAMANTANE DIOXETANE SUBSTRATES - STABLE LABELS FOR LUMINESCENCE-ENHANCED ENZYME IMMUNOASSAYS

  • 1 July 1990
    • journal article
    • research article
    • Vol. 28  (7) , 481-483
Abstract
This short communication describes the use of spiroadamantane-1,2 dioxetane substrates for alkaline phosphatase and .beta.-D-galactosidase in the development of luminescence-enhanced enzyme immunoassays. Until the present work, only peroxidase-luminol/peroxidase systems had been used in such assays. The light reaction kinetics were studied from the time of initiation of the light reaction up to 2 days later. The method is illustrated by the determination of myeloperoxidase, based on microtitre plate technology, coupled with a microtitre plate luminometer which needs no injector. The alkaline phosphatase and peroxidase systems were compared.

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