Identification of introgressed segments conferring disease resistance in a tetrageneric hybrid of Triticum, Secale, Thinopyrum, and Avena

Abstract
Using genomic in situ hybridization to chromosomes, we identified introgressed segments in a tetrageneric hybrid of Triticum, Avena, Thinopyrum, and Secale, which conferred high resistance to leaf rust, stem rust, stripe rust, powdery mildew, and root rot to wheat. The disease-resistance traits of the hybrid originated from three wild related genera of Triticum, namely Avena, Thinopyrum, and Secale. The new breeding system that combined traditional wide hybridization with anther culture was efficient and rapid in creating wheat germplasms resistant to major diseases.Key words: Triticum aestivum, Avena fatua, Thinopyrum intermedium, Secale cereale, wide hybridization, anther culture, genomic in situ hybridization, GISH.

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