Effect of Detergents and Acid Buffer on the Reactivity of Fasciola hepatica Antigens

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Abstract
The effect of glycine HCl (acid) buffer on antigen extraction and of 3 commonly used detergents, sodium dodecyl sulfate (SDS), sodium deoxycholate (DOC) and Triton X-100, on the reactivity of a standard E. hepatica adult-worm antigen preparation (FhWWE) was studied. Acid buffer destroyed most of the immunologic reactivity of FhWWE when tested with anti-F. hepatica serum, but apparently did not affect the antigens reactivity with anti-Schistosoma mansoni serum. Elution with acid buffer should be used with caution when FhWWE is the antigenic extract used in antibody-affinity chromatography. Neither Triton X-100 nor DOC appreciably increased the reactivity of FhWWE antigens. DOC decreased the antigen-antibody interactions. Low concentrations of SDS increased the reactivity of FhWWE, but higher concentrations decreased the antigen-antibody interactions.