Chemical factors in the germination of spore-bearing aerobes. The effect of yeast extract on the germination of Bacillus anthracis and its replacement by adenosine

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Abstract
The metabolites essential for the rapid germination of spores of B. anthracis were studied, using loss of resistance to heat as a measure of germination, detd. by viable count. A suitable deficient medium for the assay of promoters of germination contained 0.25% acid-hydrolyzed gelatin and 0.005% L-tyrosine. Higher concns. of hydrolyzed gelatin were inhibitory. The slow germination in the basal medium was accelerated significantly by yeast extract equivalent to 0.01% fresh yeast. The yeast extract was replaceable by a purified prepn. of adenosine which gave a significant effect at a concn. of 0.2 [mu]g./ml.
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