SEROLOGICAL AND PHYSICAL PROPERTIES OF SOME STONE-FRUIT VIRUSES: I. PREPARATIVE AND SEROLOGICAL TECHNIQUES

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Abstract
Strain Y.4 of the cherry yellows virus was purified from cucumber by four methods, which differed mainly at the clarification stage: A, the homogenate buffered at pH 6.5 was frozen; B, leaves were frozen and the expressed sap was buffered at pH 5.0; C, fresh expressed sap was diluted with pH 5.0 buffer; D, fresh expressed sap was dialyzed against.05 M acetate buffer at pH 4.5. One or two cycles of differential centrifugation were applied after clarification. Freezing usually inactivated the virus. Methods C and D yielded infective preparations. Ultraviolet absorption spectrum analysis, and particle size and sedimentation rate determinations, supported by infectivity tests, indicated that, in these infective preparations, the virus replaced most if not all of the components found in comparable extracts from healthy sources. The agar gel diffusion technique was used for precipitin tests. Virus antigen from cucumber sources reacted with homologous antiserum or with antiserum derived from infected cherry petals and vice versa.

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